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A non-heme ferric dioxygenase catalyzing the intradiol cleavage of all the examined catechol derivatives, 3,5-dichlorocatechol, was reported in Pseudomonas cepacia CSV90, grown with 2,4-D as the sole carbon source. is reported to produce a high molecular weight C12O (100 kDa) that is stable at pH 8 but optimally active at pH 6.2. Pseudomonas aeruginosa TKU002 capable of mineralizing benzoic acid was reported to produce a low molecular weight C12O showing the highest activity against pyrogallol, which is an unusual characteristic. found to be dependent on Fe 2+ and Fe 3+ ions with high substrate specificity, showing molecular weight ranges from 22 to 35 kDa. Ĭ12O was first isolated and purified in Pseudomonas spp. C12O contains Iron(III) oxide as a prosthetic group, and it is part of the enzymes that cleave catechol via the ortho-cleavage, resulting in the formation of cis-cis-muconic acid. C12O is mostly reported in Gram-negative bacteria, but much less information is available about these enzymes in Gram-positive bacteria. The enzyme incorporates an oxygen atom into the catechol, resulting in the formation of cis-cis-muconic acid. Ĭatechol 1,2-dioxygenases have the potential to be used in the process of remediating wastewater contaminated with phenol, benzoate, fluorocatechol, bromocatechol, chlorocatechol, methylcatechol, herbicides (diuron), polychlorinated biphenyls, and chloroethanes. The final intermediates of both pathways then enter the tricarboxylic acid cycle. The catechol is then oxidized via an ortho-cleavage pathway by catechol 1,2-dioxygenase (C12O), or via a meta-pathway to 2-hydroxymuconic semialdehyde by catechol 2,3-dioxygenase (C23O) to open the ring. During the aerobic biodegradation of aromatic compounds, phenol, and it derivatives (e.g., 2,4-dichlorophenoxacetic acid (2,4-D) and 2,4-dichlorophenol (2,4-DCP)), catechols are formed as the central intermediates by the introduction of hydroxyl groups facilitated at ortho- or meta-positions. Microorganisms have developed mechanisms to degrade these compounds with the aid of enzymes. The widespread distribution of aromatic compounds in the environment has led to an increase in pollution, which affects the health quality of living organisms. The characterization and optimization of C12O activity can assist in understanding the 2,4-DCP metabolic pathway in PcUFB2 and its possible application in bioremediation strategies. ES-MS spectra of tryptic digested SDS-PAGE band and bioinformatics studies revealed that C12O shared 81% homology with homogentisate 1,2-dioxygenase reported in other Pseudomonas chlororaphis strains. The Lineweaver–Burk plot showed the v max and K m values of 16.67 µM/min and 35.76 µM, respectively. The enzyme was optimally active at pH 7.5 and a temperature of 30 ☌. A 35 kDa protein was purified to 1.5-fold with total yield of 13.02% by applying anion exchange and gel filtration chromatography. The crude cell extract showed 10.34 U/mL of C12O activity with a specific activity of 2.23 U/mg of protein. Preliminary studies showed that PcUFB2 could degrade 40 ppm of 2,4-dichlorophenol (2,4-DCP). The aim of this study was to purify, characterize, and predict the template-based three-dimensional structure of catechol 1,2-dioxygenase (C12O) from indigenous Pseudomonas chlororaphis strain UFB2 ( PcUFB2). Catechol dioxygenases in microorganisms cleave catechol into cis- cis-muconic acid or 2-hydroxymuconic semialdehyde via the ortho- or meta-pathways, respectively. Just keep in mind that mechanical enemies are weak against energy and pulse weapon types. Since you’re able to build as many soldiers as you want, you may as well add a bit of variety to the bunch. You will want to hunt down a lot of robots though, as it will grant you way more customization options in the long run. So don’t waste your time trying to farm missions where you know you fight a lot of bots. Robots that existed before the DLC (regular Synths, Assaultrons, Protectrons, etc.) won’t drop any. #Best automatrons mods#They will always drop mods for you to collect, and once you collect a mod, you will be able to build it as many times as needed (provided that you have the resources, of course). Mods are found easily, you just need to kill any of the new robots added by the Automatron expansion. These will allow you to build the parts that help to define your robot compatriots, and build some horrific defenders. To build something tougher, you’ll need mods. In the beginning, you’ll be able to build some basic, but useful, Automatrons to defend you, but you’ll need something much more special in the long run. That workbench isn’t just for show, you are to build the best and baddest mechanical fighters this side of the nuclear explosion that destroyed your world. Thankfully, Automatron’s positives are enough to make it a solid opening jaunt into Fallout 4’s future.Once you get past the opening mission of Automatron, Fallout 4’s first major expansion, and build your very first Robot Workbench, it’s time to get to work. It’s to be expected with given the main game, maybe, but that isn’t an excuse. It crashed for me on two boss fights, and the usual visual anomalies persisted as robot parts floated in mid-air or vibrated around the floor after a fire fight. Luckily, the robot crafting and new enemies are enough to carry Automatron above a smattering of bugs and glitches that bleed in from the main game. At a certain point you’ll need to reassure her that it wasn’t her fault her companions died and from then on, she’s kind of okay with it. Ada herself is conflicted enough to be interesting, but only just. Eventually, the writers will need to come up with something new. It’s nothing we haven’t seen before in the main game, and shines a light on the fact that eccentric characters only carry you so far. The boss fight is decent, but his character is by-the-numbers, as is snarky “Robo-brain” Jezebel, whose body you need to build as part of the story so that she’ll help you find and defeat the enemy. The Mechanist himself is fairly disappointing, too. The story itself lacks any real pizzazz, content to simply give you a new villain and ask you to complete some busy work until it’s time to kill him. The robot crafting is the icing on the cake, but it’s not all sweet to the taste. The missions are standard fare and don’t add much beyond the robot baddies, but you’ll play it to conclusion just because it’s more of what you like. I died a few times on some of the tougher encounters, especially as they also throw a new bandit type at you in the form of the Rust Devils, human enemies who use robots like attack dog and wear their scavenged body parts as armour. The first few times they will take you by surprise, as it’s something you’re just not used to seeing in Fallout 4. The enemy variety is decent, introducing a good mix of ranged attackers and a new robot that will literally run at you an leap on you. In short, you get them by destroying the psychotic murder-bots that now stalk he Commonwealth thanks to the Mechanist. How you come by these components is just as fun. You can even add a lick of paint and choose the voice. Your robot can walk, travel on caterpillar tracks or hover you can outfit it with blades, guns, laser cannons, flamethrowers or claws there are numerous head, limb and torso combinations. After creating a base model, you can make a huge number of adjustments. This being Fallout 4, the options are impressive as long as you have the necessary components. One of the first things Ada asks you to do is build a robot workstation, which allows you to either customise your mechanical companions or create a new one from scratch. Spread across four main missions, Automatron lasts for about four hours, and I spent a large chunk of that time looking for materials for the new crafting station. The only survivor is a robot named Ada, who joins you as a companion and compels you to end the Mechanist’s tyranny and give her fallen friends peace. It begins when you follow a distress signal and find a caravan slaughtered by merciless robots controlled by a self-styled villain who likes to be known as the Mechanist. Fallout 4‘s season pass is off to an enjoyable if short-lived start with Automatron, a slice of DLC that brings new missions, new enemies, new weapons, and yet another way to distract yourself from the grimness of the Wasteland. While wallets seem to be getting smaller and the role of cash is dwindling, we simply cannot live without our bags and bag-like accessories. Today, just as the world’s best-known luxury houses are celebrated for their iconic handbags and purses, brands such as Louis Vuitton, Hermès, Gucci, Chanel and others offer a sweeping array of sophisticated wallets and small accessories for any event. Coin purses were no longer the most convenient option for carrying cash. It wasn’t until 1690 in the United States that paper money could finally be printed and used to pay taxes, for example, following the General Court of Massachusetts’s order that a limited batch be produced to pay soldiers owing to a shortage of coins.Īs paper money became more common, so too did carrying wallets. The invention of paper money dates back to the 7th century in China, but, globally, it took a while to catch on. Vintage and designer wallets and small accessories, such as wristlets, belt bags, clutches and other compact items, can be the perfect accessory for a night out or running everyday errands. Finding the Right Wallets and Small Accessories for You Find a variety of vintage Hermès handbags, day dresses, shoes and more on 1stDibs. There’s the Constance bag, a favorite of Jacqueline Kennedy, the recently relaunched 1970s-era Evelyne and, on the vintage market, a slew of designs dating back to the 1920s. Since the 1920s, the brand has produced some of the most desirable leather goods in the world. While the Kelly and Birkin may be standouts, gracing the arms of everyone from royal heiresses to hip-hop stars in the past few decades, the handbags are but a small part of Hermès’s fashion offerings. Owing to the brand’s legendary commitment to deft, handcrafted construction, the Birkin is an investment that is coveted by collectors everywhere. After the two travelers were properly introduced, Birkin helped design Jean-Louis’s most famous contribution to the Hermès canon: the Birkin bag, a roomy, square catchall with the HAC’s trademark leather flap top and the addition of a lock and key. In 1981, Robert Dumas’s son Jean-Louis Dumas, then Hermès chairman, found himself sitting next to French actress and musician Jane Birkin on a plane, where she was complaining about finding a suitable carryall for the necessary accoutrements of motherhood. Kelly even once used one as a sling for her broken arm. Well-heeled women wear it on their heads, around their necks and, in a genius piece of cross-promotion, tied to the straps of their Hermès bags. Today, vintage Hermès scarves, typically adorned in rich colors and elaborate patterns, serve many functions, just as they did back then. Based on a woodblock designed by Dumas and printed on Chinese silk, the accessory was an immediate hit. Robert Dumas was also responsible for another one of the brand’s most iconic offerings: the launch of its first silk scarf on the occasion of Hermès’s 100th anniversary in 1937. Each Kelly bag takes between 18 and 25 hours to produce, and its 680 hand stitches owe solely to one Hermès artisan. In 1977, Hermès officially renamed the model for her, and the Kelly bag was born. Because she was photographed constantly, the coverage catapulted her handbag to international popularity. Later, actress Grace Kelly, then engaged to Prince Rainier of Monaco, is said to have used one of these bags to conceal her pregnancy during the 1950s. In the 1930s, Robert Dumas (son-in-law to Émile-Maurice Hermès, Thierry’s grandson) designed a smaller, trapezoidal take on the flap bag with a handle and two side straps. Today, 120 years later, the HAC remains in Hermès’s line - and its distinctive flap and clasping straps have laid the groundwork for some of the house’s other iconic bags. As the world made the switch from horse to automobile, the bag adapted, becoming a multifunctional travel satchel instead of a designated saddlebag. A tall bag secured with a folded leather flap (fastened with bridle-inspired straps), it was designed to transport riding boots and a harness. The first top-handled bag ever produced by Hermès was the Haut à courroies, which made its debut in 1892. Gradually, the house extended into accessories and luggage for its riders, and today, in paying homage to its origins, the family-run luxury brand resurfaces horse motifs in everything from clothing and modernist jewelry to pillows and handbags. In 1837, German-born French entrepreneur Thierry Hermès opened a saddle and harness purveyor in Paris. For Hermès, what began as a maker of leather equestrian goods for European noblemen would eventually grow into one of the most storied fashion labels in the world. And thanks to these mod geniuses, you can do all that and drop several hundred simultaneous users into the mix so you're not just going against the AI. #Just cause 2 mods 360 zip#Want to drive boats, bikes, jets, and helicopters (or tie them all together with zip lines) making use of a tongue-in-cheek physics system spread across one of the largest open gaming worlds you'll ever see? That's Just Cause 2 in a nutshell. Want to pull the rip-cord on your parachute and use the car's momentum to soar over the city streets, firing from the hip at all the bad guys giving chase? You can do it as soon as you log in there's no minimum level or gear requirements for being a badass. Want to drive a sports car through a tropical urban cityscape and flip up onto the roof while it's still moving? Go ahead. In fact, as JC2's protagonist Rico, a suave secret agent with a sexy south-of-the-border accent, you'll regularly be doing things that James Bond could never imagine in a million years. Well, technically it's on consoles too, but guess what? The multiplayer mod isn't! Anyhow, the game sounds rather average at first blush (and it also sounds like a really bad Sean Connery film), but trust me, it's neither. If you haven't played it, it's an open-world, third-person action game for the PC. Fortunately I was able to take part in one of the weekend beta tests very recently, and I came away with a load of screenshots to show you just how awesome these dudes and their creation really are. The Just Cause 2 multiplayer server is a work in progress, and as the mod team says in its FAQ, the server up-times tend to be quite random (also, "don't ask"). See, as awesome as everything that I'm about to describe is, you can't actually do it right now. OK, first off let me apologize for being something of a tease. Speaking of, won't you join us after the cut for a look at the crazy fun to be had on Just Cause 2's multiplayer server? #Just cause 2 mods 360 Pc#Burnout will turn its all-seeing eye on RPGs and open-world action games primarily, and we'll do our due diligence when it comes to the PC modding community, too. MMO Burnout, then, is our latest branching opinion column, and as the title suggests, it's largely concerned with what to play when you tire of gear resets, rep grinds, and being your shard's 3,721st incarnation of "the One."ĭon't worry, though we're not delving into Call of Duty or Angry Birds. If you've been following the site for a while now, you've probably noticed us reaching into the realm of MOBAs, shooters, and mobile/browser titles. Wait, what the heck is this? MMO Burnout? On an MMO site? Yes, kids, this is the scary part of the story where Massively strays from the straight and narrow, beating a path through the tangled underbrush of pseudo-MMOs and single-player games that are nonetheless related to MMOs by way of a crucial feature or two. Var base64EncodedBytes = Convert.FromBase64String(base64String) We are going to use the output from ToBase64String() conversion for “world!”, the base64String variable: // before: d29ybGQh It converts from Base64 to its equivalent 8-bit unsigned integer array. This method is the opposite of the ToBase64String() method. Decode the Base64 String in C# With FromBase64String() We can see that the value has been broken into two lines. after: VGhlIGdyZWF0IGNyb2NvZGlsZSBvZiBRdWVlbnNsYW5kIGNhbiBhdHRhaW4gYSBsZW5ndGggb2Yg Var base64String = Convert.ToBase64String(textBytes, Base64FormattingOptions.InsertLineBreaks) Var textBytes = ("The great crocodile of Queensland can attain a length of 30 feet") Conversely, the encoding scheme will add a line break for every 76 characters. Line breaks can improve the readability of our Base64 text and also assist us while using tools that don’t deal well with long lines. We can use the options parameter to insert line breaks within our Base64 output. Using the last example, the value for this parameter was 6. We can use this parameter to state the number of elements or variables we want to encode. This parameter works hand-in-hand with the offset parameter. Var base64String = Convert.ToBase64String(textBytes, 6, 6) Similarly, the Base64 output will be different from the result we got when all the value was encoded: var textBytes = ("Hello world!") We must pair this parameter with the length option to make it work. Using our last example of “Hello world!”, if we want to encode only “world!”, we will set our optional parameter to 6. It states the position we want our encoding to start from. Var base64String = Convert.ToBase64String(textBytes) Bitcoin signature malformed base64 encoding code#Without tracking down the code this calls, that looks like it should recover and encode the pubkey whether it also verifies the signature is not obvious.Var textBytes = ("Hello world!") $derPubKey = Signature::getPubKeyWithRS($flag, $R, $S, $hash) That correctly parses a plain-format signature (not DER), from base64. $flag = hexdec(bin2hex(substr($signature, 0, 1))) The code you link to appears correct: $signature = base64_decode($encodedSignature) Your signature should not be and is not in DER format. The computation of the r,s values in an ECDSA (which as Oscar correctly commented are not a point) is the same either way, but the data being signed for a message can never be the same as for a transaction so the signatures can never actually be the same value. I try alot of methods but no luck, are R/S signature in both signed message and DER signature means to same?īitcoin transactions use DER format for the signature (plus sighash byte), but bitcoin messages use 'plain' format (aka P1363, CVC, PKCS11, Microsoft, JWS and more) (plus recovery byte). If another party send me DER signature, how do i convert it into base64_encoded string? so end up i am able to use function i mention just now. My program check valid signature using function in I realized that this DER signature is fail in signature verification. after that, i use R/S signature to generate DER signature. Then i manage to get R/S signature from $decoded_content. My working way is $decoded_content = bin2hex(base64_decode(IPnTEOU713+XclrBrUEfTLeP0A7QIR4lObzQjBjyxGQExPwkalrQK1JOggIWk4xrfWIjHEC0RKGv3vtguDfto0o=)) IPnTEOU713+XclrBrUEfTLeP0A7QIR4lObzQjBjyxGQExPwkalrQK1JOggIWk4xrfWIjHEC0RKGv3vtguDfto0o= i realize that R/S signature in DER signature is not match to the 1 in bitcoin signed message? Ok below is my say signed message. This time is difficult for me to explain problem i was facing. DirecTV 20 Best Episodes Of Undercover BossĮpisode 3, Season 2 of Undercover Boss was a very exciting one. Overall, it was a very heartwarming episode. For instance, when a repairman uses sandpaper instead of a sandblaster or a front desk attendant logs information on paper instead of using a digital medium.ĭespite the drawbacks, he enjoyed his time with the employees very much and even gave his employees gifts for their hard work and dedication. Many of the employees still reverted to the old ways that consume more time and energy. He discovered his employees were not properly trained and lacked communication skills. Cloobeck as Jack would tail four different employees of his resort and understand how the internal workings of his company happen. Jack wanted to quit his day job and experience the world of hospitality. He is the owner of one of the biggest hotel chains in the USA. Cloobeck, CEO of Diamond Resorts International. Read Also: One Punch Man Webcomic Vs Manga: Which Is Better? Diamond Resorts International 20 Best Episodes Of Undercover Boss Fans were very impressed by his quick and efficient decision-making and applauded him for it. The restaurant opened the next morning with a new general manager. However, he did not close the branch permanently. Horrified by his behavior, Rick immediately shut down the branch and sent Stevens for further training. In his defense, Stevens said if he didn’t shout the employees would not listen to him. When Rick witnessed this behavior, he immediately confronted him. So what happened? One of the managers of that branch known as ‘Stevens’ not only shouted and misbehaved with employer Todd, he even proceeded to beat him. Things escalated so quickly that Rick broke character and even shut down the branch then and there. He discovers everything wrong with his restaurant starting from food preparation, management, and training. What he found in one of the branches ofĬheckers & Rally’s drive-in hamburger restaurants were shocking. CEO Rick Silva went undercover as Alex Garcia, a failed pharmacy owner from Philadelphia. Checkers and Rally’s 20 Best Episodes Of Undercover BossĮpisode 4 of Season 3 is undoubtedly very interesting. It is a really fun episode and fans loved it. In between all the heavy work, he understands how hard his employees work. Unable to cope with the daily workings of the company, he even reveals himself unexpectedly. Even though he is excellent at running such a huge company, he struggles to hang a dry well. But how does this big company function on the inside? To find out CEO, Sheldon Yellen goes undercover. Belfor is a disaster restoration company and also the best in the world. Best undercover boss episodes series#Fans love this series because it is fun and refreshing. Number one on this list of 20 best Undercover Boss episodes is episode 14 of season 2. Belfor 20 Best Episodes Of Undercover Boss So let’s get into the list of the top 20 best episodes. It is the best way to learn about all the things that need improvements and the demands the employees have. CEOs, founders, and presidents of big companies go undercover and learn about the workings of their companies. And Undercover Boss focuses on just that. The best way is to get down and dirty in the field. Running a successful company does not only mean sitting in an office and giving orders. #Magic trail clear background professional#Ginobili accomplished his feats with flair, fearlessness, dogged competitiveness, selflessness and reckless abandon over a 23-year career in professional basketball, which included a 16-season run with the Spurs that produced four titles.īut there’s so much more to the man, according to former teammate Brent Barry, who now serves as San Antonio’s vice president of basketball operations. When Duncan introduces Ginobili as the headliner of the Naismith Hall of Fame Class of 2022 on Saturday, you’ll likely hear countless tales of the ultra-competitiveness that pushed the Argentine to the mantle of winningest player in NBA history (72.1%) among those appearing in at least 1,000 games. “He doesn’t know how, and that’s his strength.” “The most competitive player we’ve ever had,” Spurs CEO R.C. Manu Ginobili celebrates the Spurs’ championship in 2014. The heart-stopping process, like so many times before, only repeated itself the very next day in another inconsequential pickup run when Ginobili dove through the legs of franchise stalwart Tim Duncan to scoop up a loose ball. He blocked the dunk right there at the rim. “Every reason to do it was wrong, and he did it. #Magic trail clear background free#“You’re like, ‘Bro, he is not about to try to make a play at the rim two weeks before training camp, on a free agent,’ ” Hardy said. Like everybody else in the gym that day, Hardy cringes in anticipation of what might happen next. That’s when Hardy (then a Spurs assistant coach under Gregg Popovich) spots a figure out the corner of his eye bearing down in hot pursuit, as the sound of squeaking sneakers fades into the horrid silence of the moment. In a routine pick-up game, Mario West - an unsigned and unheralded free-agent - sprints the floor in transition for what should be an easy breakaway dunk. By now, the veteran owns an Olympic gold medal and three NBA championship rings, not to mention a pair of All-Star nods and an NBA Sixth Man of the Year Award. It’s summer 2012, and Manu Ginobili has just turned 34. “You could just see it happening,” Utah Jazz coach Will Hardy explained to NBA.com. SAN ANTONIO – The split-second buildup induced butterflies, sending chills through a steamy gym two weeks before the start of training camp. Manu Ginobili talks with former Spurs assistant coach Will Hardy prior to a game in 2018.
The Arc's own status light consists of LEDs that shine through four holes above the subtle Sonos logo (fun fact: there are 76,000 holes in all the grilles combined). At least it could were it not for the integrated IR repeater, which is enabled by default and works flawlessly during testing. That's unlikely to be so high as to encroach on your TV's picture, but it could conceivably block the signals being sent to it by your remote control. The curvy design helps mask the Arc’s dimensions and makes it look svelte, but its height measurement of 8.5cm is not inconsiderable. Each end also features a grille-covered driver. Once in position, most of what you can see of the Arc is a 273-degree grille that hides the array of front-mounted and up-firing speakers. The bespoke Sonos wall mount is a £69 / $79 / AU$99 optional extra but there are also now plenty of third-party Arc wall mounts available for less. #Ikea pricetracker softwareIf you don't have a sideboard big enough to accommodate its sizeable width, the Arc can be wall-mounted, with onboard Trueplay tuning software automatically adjusting the speaker's frequency response accordingly. Voice control Amazon Alexa, Google Assistantĭimensions (hwd) 8.7 x 114 x 12cm / 3.4 x 45 x 4.5 inches This app also allows you to see live price drops, so you can check if the price of an item falls in real time.Surround format support Dolby Atmos, Dolby True HD, Dolby Digital+, Dolby Digital, DTS It supports the major retailers, including Amazon, Target, Ikea, Fry’s, HomeDepot and many more. This web app has add-ons for Firefox, Chrome and Safari. PriceZombie allows your business to budget more carefully by tracking prices and price histories while making sure the item you want to buy is the best option for your business and purposes. You can even earn cashback when shopping with ShopSavvy. Each day the app collects millions of products from shopping websites and sorts through the prices and sales to bring your business the best deals. Your purchasing team can use this app to discover new products and shopping trends and find the best online and in-store deals from hundreds of the most popular retailers, including Amazon, Walmart, Target, Newegg and Best Buy. ShopSavvy is a price comparison and barcode scanner app available for iPhones and iPads on the App Store for free. #Ikea pricetracker androidThe Smartprix price comparison app is compatible with all Android devices and is free to download. #Ikea pricetracker plusYou can search and browse more than 10 million products from 100 plus categories and get instant price alerts with the Smartprix app. Your buying team can also watch prices on virtually any item available on the major shopping sites. The Smartprix app is a great tool for letting you know of up-to-the-minute discounts and deals around the internet. #Ikea pricetracker downloadPrice Tracker for Amazon is free to download on all Android devices from Google Play. Notifications can be set to either be generated when lower prices are detected or when items drop to or below a specified price target. The app alerts you when your desired items drop in price through ultra-fast notifications. If your business makes a lot of purchases on Amazon, Price Tracker for Amazon will help you save money by tracking and checking the prices of the products you need to buy. Give those responsible for buying products in your business a helping hand with the following 10 best price tracking apps for small business owners. |
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